首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子内皮素1(ET-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EDN1含量。EDN1即内皮素 -1，是一种具有强大而持久血管收缩作用的多肽。在人体生理病理过程中地位重要，与心血管疾病、肿瘤等多种疾病的发生发展相关。研究聚焦于其信号转导机制、与受体相互作用及在疾病中异常表达调控机制，以助开发相关治疗策略。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于心血管疾病机制研究、内皮功能评估、药物干预效果分析等基础科研领域，支持在动物模型或离体实验中定量分析ET-1表达动态，为血管生物学、病理生理学等研究提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
缩写：EDN1
Uniprot No.：P29560
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 98 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.6302.4562.5432.4582002.1112.0762.0942.0091001.4591.4431.4511.366500.8160.7690.7930.708250.4150.3890.4020.31712.50.2180.2030.2110.1266.250.1470.1490.1480.06300.0880.0820.085 | ||||||
货期：3-5 working days
×
