首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：大鼠雌二醇受体(ER)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ESR1含量。ESR1 即雌激素受体 1，在人体生理过程中扮演重要角色，尤其与雌激素信号传导密切相关。其异常表达或功能失调与多种疾病，特别是乳腺癌的发生发展紧密相连。研究多聚焦于其作用机制及靶向干预，旨在开发更有效的治疗手段。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于研究雌激素相关信号通路、评估环境激素干扰物对生物体的影响、探索妇科疾病或骨质疏松的分子机制等科研领域，为实验室开展激素受体动态监测、药物作用靶点验证等研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Esr1 ELISA Kit; Esr ELISA Kit; Estr ELISA Kit; Nr3a1 ELISA Kit; Estrogen receptor ELISA Kit; ER ELISA Kit; ER-alpha ELISA Kit; Estradiol receptor ELISA Kit; Nuclear receptor subfamily 3 group A member 1 ELISA Kit
缩写：ESR1
Uniprot No.：P06211
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 86-91 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 81-95 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of rat ER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 94-109 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.9152.7312.8232.70010002.2212.1772.1992.0765001.3741.4551.4151.2922500.7860.8100.7980.6751250.4880.4960.4920.36962.50.3240.3400.3320.20931.250.1930.1870.1900.06700.1190.1260.123 | ||||||
货期：3-5 working days
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