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产品描述:人雌二醇受体(ER)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ESR1含量。ESR1(雌激素受体1)是一种核激素受体,在雌激素依赖性恶性肿瘤中发挥关键作用。其突变会导致肿瘤细胞增殖、上皮-间质转化和转移,是乳腺癌内分泌治疗耐药的重要机制。研究ESR1的机制有助于开发新的治疗策略,提高治疗效果。试剂盒检测范围为3.12 pg/mL-200 pg/mL,该产品适用于体外科研实验,可广泛用于探究ER在激素调控机制、肿瘤发生模型中的动态变化,以及药物干预或基因编辑后ER表达水平的定量评估,为雌激素相关信号通路研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:7*/654 isoform ELISA Kit; 7*/819 2 isoform ELISA Kit; 7*/822 isoform ELISA Kit; 8*/901 isoform ELISA Kit; 8*/941 isoform ELISA Kit; DKFZp686N23123 ELISA Kit; ER alpha ELISA Kit; ER ELISA Kit; ER-alpha ELISA Kit; Era ELISA Kit; ESR ELISA Kit; ESR1 ELISA Kit; ESR1_HUMAN ELISA Kit; ESRA ELISA Kit; Estradiol receptor ELISA Kit; Estrogen nuclear receptor alpha ELISA Kit; Estrogen receptor 1 ELISA Kit; Estrogen receptor alpha 3*,4,5,6,7*/822 isoform ELISA Kit; Estrogen receptor alpha ELISA Kit; Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform ELISA Kit; Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform ELISA Kit; Estrogen receptor alpha delta 4 +49 isoform ELISA Kit; Estrogen receptor alpha delta 4*,5,6,7*/654 isoform ELISA Kit; Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform ELISA Kit; Estrogen receptor alpha E1 E2 1 2 ELISA Kit; Estrogen receptor alpha E1 N2 E2 1 2 ELISA Kit; Estrogen receptor ELISA Kit; ESTRR ELISA Kit; NR3A1 ELISA Kit; Nuclear receptor subfamily 3 group A member 1 ELISA Kit
缩写:ESR1
Uniprot No.:P03372
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:3.12 pg/mL-200 pg/mL
灵敏度:0.78 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 92 | |||||
Range % | 88-95 | ||||||
1:2 | Average % | 95 | |||||
Range % | 90-100 | ||||||
1:4 | Average % | 96 | |||||
Range % | 90-102 | ||||||
1:8 | Average % | 101 | |||||
Range % | 96-106 |
回收率:
The recovery of human ER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 90 | 87-95 | |||||
EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 2002.6342.6472.6412.451 1001.9351.8771.9061.716 501.1421.2361.1890.999 250.7360.7870.7620.572 12.50.4470.4540.4510.261 6.250.3110.3250.3180.128 3.120.2400.2510.2460.056 00.1890.1910.190 . |
货期:3-5 working days
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