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Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit

  • 中文名： 人内皮细胞特异分子-1(ESM-1)ELISA Kit
货号: IPD-E16530h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人内皮细胞特异分子-1(ESM-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆s.样本中的ESM1含量。试剂盒检测范围为3.12 ng/ml-200 ng/ml，灵敏度为0.78 ng/ml。可广泛应用于心血管疾病机制、肿瘤微环境研究、炎症相关疾病模型等科研领域，为探索ESM - 1在血管内皮功能调控、肿瘤血管新生等过程中的作用提供可靠工具，助力生物标志物筛选及分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Endocan ELISA Kit; Endothelial cell specific molecule 1 ELISA Kit; Endothelial cell-specific molecule 1 ELISA Kit; ESM 1 ELISA Kit; ESM 1 secretory protein ELISA Kit; ESM-1 ELISA Kit; Esm1 ELISA Kit; ESM1 secretory protein ELISA Kit; ESM1_HUMAN ELISA Kit
  • 缩写：
    ESM1
  • Uniprot No.：
    Q9NQ30
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenatess.
  • 检测范围：
    3.12 ng/ml-200 ng/ml.
  • 灵敏度：
    0.78 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Tags & Cell Markers
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ESM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    100


    Range %

    96-104


    1:2

    Average %

    106


    Range %

    102-112


    1:4

    Average %

    97


    Range %

    90-104


    1:8

    Average %

    101


    Range %

    97-105


  • 回收率：

    The recovery of human ESM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    99-105


    EDTA plasma (n=4)

    98

    91-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    200

    1.674

    1.634

    1.654

    1.464


    100

    1.275

    1.247

    1.261

    1.070


    50

    0.983

    1.028

    1.005

    0.815


    25

    0.727

    0.739

    0.733

    0.543


    12.5

    0.595

    0.621

    0.608

    0.417


    6.25

    0.440

    0.453

    0.447

    0.256


    3.12

    0.316

    0.304

    0.310

    0.120


    0

    0.186

    0.195

    0.191



  • 本试剂盒所含材料：



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ESM1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled ESM1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.


    • 货期：
      3-5 working days

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