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产品描述：人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ERVK6含量。人HERV-K_7p22.1前病毒祖先Env多蛋白（ERVK6）是内源性逆转录病毒K组的一个成员，其Env蛋白在早期感染中参与受体识别和膜融合。ERVK6蛋白在血清、血浆和组织匀浆中定量测量，具有潜在的研究价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外样本中ERVK6蛋白表达水平的精准分析，可为病毒进化研究、宿主基因组稳定性探索以及相关疾病模型的分子机制解析提供技术支持。该产品严格适用于科研场景，包括但不限于细胞实验、动物模型或生物样本库中ERVK6蛋白的动态监测，为分子生物学及病毒学领域的研究者提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
缩写：ERVK6
Uniprot No.：Q69384
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 110 | |||||
| Range % | 106-114 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 81-89 | |||||
| EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.2512.3012.2762.15451.3751.3681.3721.2502.50.7820.7780.7800.6581.250.5030.5110.5070.3850.6250.3250.3360.3310.2090.3120.2470.2680.2580.1360.1560.1920.1860.1890.06700.1210.1230.122 | |||||||
货期：3-5 working days
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