首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠内皮抑素(ES)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ES含量。大鼠内皮抑素（ES）是一种血管生成抑制因子，通过抑制内皮细胞增殖和迁移，发挥抗血管生成作用，促进肿瘤细胞凋亡。其研究机制涉及抑制VEGF表达、抑制新生血管生成，并可能通过血管“正常化”作用提高放疗敏感性。试剂盒检测范围为6.25 ng/mL-400 ng/mL，在科研领域，本产品可广泛应用于癌症模型血管抑制效应评估、心血管疾病机制研究、抗血管生成药物开发等基础研究场景，为探究ES在病理生理过程中的动态变化及其调控网络提供标准化工具，助力血管生物学、肿瘤学等领域的基础科研工作。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP
缩写：ES
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of rat ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-98 | ||||
| EDTA plasma (n=4) | 95 | 92-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.5312.4542.4932.3412001.8891.7771.8331.6811001.2081.1971.2031.051500.7040.6870.6960.544250.4270.4340.4310.27912.50.3110.3270.3190.1676.250.2390.2420.2410.08900.1500.1540.152 | ||||||
货期：3-5 working days
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