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产品描述：人ERBB受体反馈抑制剂1(ERRFI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ERRFI1含量。ERRFI1是一种细胞质蛋白，其表达随细胞生长而上调，与大鼠基因33的蛋白质产物具有显著同源性。在细胞应激期间被诱导，介导细胞信号传导，具有SH3结构域结合活性、激酶结合活性和小GTP酶结合活性，参与细胞对生长因子和激素的响应以及大分子代谢过程的负调控。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒适用于基础科研中探究ERRFI1在肿瘤信号通路中的作用机制、评估靶向治疗模型的分子响应，或通过体外细胞实验及动物模型分析其在特定生理病理条件下的动态表达，为癌症相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ERBB receptor feedback inhibitor 1 ELISA Kit; ERRFI_HUMAN ELISA Kit; Errfi1 ELISA Kit; GENE 33 ELISA Kit; Gene 33; rat; homolog of ELISA Kit; Mig-6 ELISA Kit; MIG6 ELISA Kit; mitogen inducible gene 6 ELISA Kit; mitogen inducible gene 6 protein ELISA Kit; Mitogen-inducible gene 6 protein ELISA Kit; RALT ELISA Kit; receptor-associated late transducer ELISA Kit
缩写：ERRFI1
Uniprot No.：Q9UJM3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ERRFI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 83-93 | ||||||
回收率：
| The recovery of human ERRFI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-102 | |||||
| EDTA plasma (n=4) | 89 | 86-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20001.8531.9161.8851.77110001.3871.4521.4201.3065000.8750.9180.8970.7832500.5520.5770.5650.4511250.3520.3610.3570.24362.50.2460.2530.2500.13631.250.1670.1730.1700.05600.1130.1150.114 | |||||||
货期：3-5 working days
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