首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管内皮钙粘蛋白(VE-cad)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CDH5含量。CDH5即钙黏蛋白 5，是一种细胞黏附分子。其在维持血管内皮细胞间连接完整性等方面有重要作用。研究发现它参与血管生成、肿瘤转移等机制，通过调节细胞间信号传导和黏附力等，成为血管相关疾病及肿瘤治疗潜在靶点。试剂盒检测范围为3.9 ng/mL-250 ng/mL，灵敏度为0.98 ng/mL。在心血管疾病、肿瘤血管新生及炎症反应等研究中具有重要价值。本产品可为血管生物学研究、内皮功能障碍机制探索、抗血管生成药物开发等科研领域提供可靠的数据支持，尤其适用于体外实验模型中内皮细胞功能评估及相关病理生理学研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CDH5; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD antigen CD144
缩写：CDH5
Uniprot No.：P33151
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 ng/mL-250 ng/mL
灵敏度：0.98 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VE-cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:20 | Average % | 93 | |
| Range % | 90-98 | ||
| 1:40 | Average % | 95 | |
| Range % | 89-100 | ||
| 1:80 | Average % | 98 | |
| Range % | 85-104 | ||
| 1:160 | Average % | 93 | |
| Range % | 87-99 |
回收率：
| The recovery of human VE-cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-102 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2502.9782.8962.9372.719 1252.4562.3922.4242.206 62.51.6931.7041.6991.481 31.251.0411.0591.0500.832 15.60.6690.6780.6740.456 7.80.4780.4610.4700.252 3.90.3140.3350.3250.107 00.2170.2180.218 | ||||||
货期：3-5 working days
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