首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人H-钙粘着蛋白/H-钙粘连蛋白(H-Cad/CDH13)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的CDH13含量。CDH13是一种钙黏蛋白超家族成员，在细胞黏附、迁移等过程有重要作用。在研究机制上，它涉及多条信号通路，如Wnt等。其异常表达与肿瘤发生发展、心血管疾病等密切相关，被视为疾病诊断和治疗的潜在靶点。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于基础科研中CDH13蛋白表达水平分析，例如探究其在肿瘤微环境中的调控作用、心血管内皮功能研究或神经退行性疾病模型中黏附分子动态变化等方向，为疾病机制研究及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CAD13_HUMAN ELISA Kit; Cadherin-13 ELISA Kit; CDH13 ELISA Kit; CDHH ELISA Kit; H-cadherin ELISA Kit; Heart cadherin ELISA Kit; P105 ELISA Kit; T cad ELISA Kit; T Cadherin ELISA Kit; T-cad ELISA Kit; T-cadherin ELISA Kit; Truncated cadherin ELISA Kit
缩写：CDH13
Uniprot No.：P55290
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human H-Cad/CDH13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 93 | |
| Range % | 86-97 | ||
| 1:2 | Average % | 92 | |
| Range % | 89-96 | ||
| 1:4 | Average % | 102 | |
| Range % | 99-105 | ||
| 1:8 | Average % | 94 | |
| Range % | 87-99 |
回收率：
| The recovery of human H-Cad/CDH13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-102 | ||||
| EDTA plasma (n=4) | 102 | 97-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 802.3672.2352.3012.169 401.7561.8161.7861.654 201.2861.2241.2551.123 100.8150.8010.8080.676 50.4640.4530.4590.327 2.50.3230.3120.3180.186 1.250.2280.2350.2320.100 00.1330.1310.132 | ||||||
货期：3-5 working days
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