Human Neural -Cadherin, N-Cad ELISA kit

大包装询价

货号: IPD-E09718h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人N钙黏蛋白/神经钙黏蛋白(N-Cad)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CDH2含量。CDH2是重要靶点。它是一种钙黏蛋白，在细胞黏附、迁移等过程起关键作用。研究发现其异常表达与肿瘤发生、发展及转移密切相关，机制涉及调节细胞信号通路、影响肿瘤微环境等，有望为肿瘤治疗等提供新方向。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于体外研究中对N-Cad蛋白表达的定量分析，可广泛应用于肿瘤生物学研究、神经退行性疾病模型构建、干细胞分化调控等科研领域，为探索细胞黏附机制及其病理生理学意义提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CDH2; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD antigen CD325
缩写：
CDH2
Uniprot No.：
P19022
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
6.25 ng/mL-400 ng/mL
灵敏度：
1.56 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human N-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-100
1:2	Average %	97
	Range %	91-104
1:4	Average %	97
	Range %	92-108
1:8	Average %	94
	Range %	85-98

回收率：

The recovery of human N-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-105
EDTA plasma (n=4)	97	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
400	2.152	2.174	2.163	2.005
200	1.832	1.824	1.828	1.670
100	1.220	1.251	1.236	1.078
50	0.926	0.974	0.950	0.792
25	0.574	0.543	0.559	0.401
12.5	0.406	0.426	0.416	0.258
6.25	0.335	0.312	0.324	0.166
0	0.152	0.164	0.158

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.