首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胞苷脱氨酶(CDA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CDA含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探索CDA在肿瘤微环境中的表达调控、免疫细胞代谢重编程机制，或用于评估体外模型中基因编辑、药物干预对CDA活性的影响等科研用途，为肿瘤生物学、药物代谢及免疫调控研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CDA ELISA kit; CDDCytidine deaminase ELISA kit; EC 3.5.4.5 ELISA kit; Cytidine aminohydrolase ELISA kit
缩写：CDA
Uniprot No.：P32320
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CDA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-92 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-110 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human CDA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-100 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10001.8901.9521.9211.7665001.3381.3161.3271.1722500.9240.9040.9140.7591250.5850.5990.5920.43762.50.4020.4240.4130.25831.250.3210.3090.3150.16015.60.2380.2290.2340.07900.1530.1570.155 | |||||||
货期：3-5 working days
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