首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠E钙黏蛋白/上皮钙黏蛋白(E-Cad)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CDH1含量。CDH1是重要的细胞粘附分子，其编码产物与钙粘蛋白相关，在维持细胞间连接及组织形态方面起关键作用。研究表明，CDH1功能异常可致细胞粘附力降低、极性丧失，与肿瘤发生发展、侵袭转移机制密切相关，是肿瘤研究的重要靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于细胞生物学研究中上皮屏障功能评估、肿瘤侵袭转移相关机制探索、药物干预对细胞黏附特性影响等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cdh1Cadherin-1 ELISA kit; Epithelial cadherin ELISA kit; E-cadherin ELISA kit; Uvomorulin ELISA kit; CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] ELISA kit
缩写：CDH1
Uniprot No.：Q9R0T4
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 85-90 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 99 | |||||
| Range % | 95-103 | ||||||
| 1:8 | Average % | 83 | |||||
| Range % | 80-86 | ||||||
回收率：
| The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 101-109 | |||||
| EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.7502.7982.7742.5911002.0451.9682.0071.824501.4011.3581.3801.197250.9200.9670.9440.76112.50.5760.5980.5870.4046.250.4450.4680.4570.2743.120.3070.3120.3100.12700.1820.1830.183 | |||||||
货期：3-5 working days
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