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产品描述：人T细胞表面触觉蛋白(CD96)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD96含量。CD96是一种重要靶点。它属于免疫检查点分子，在肿瘤免疫逃逸等方面有潜在作用。研究机制上，其与配体结合后会影响免疫细胞活性，干扰免疫信号传导，目前针对CD96开展的研究有望为肿瘤免疫治疗开辟新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外实验中对CD96蛋白表达的动态监测，例如肿瘤微环境研究、免疫细胞功能评估或抗体药物作用机制探索等领域。本试剂盒为研究CD96在免疫调控网络中的功能及其与疾病关联性提供了可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD96; T-cell surface protein tactile; Cell surface antigen CD96; T cell-activated increased late expression protein; CD antigen CD96
缩写：CD96
Uniprot No.：P40200
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD96 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 104 | |||||
| Range % | 100-112 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of human CD96 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 93 | 88-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.0451.9722.0091.83651.5021.5811.5421.3692.51.1041.0541.0790.9061.250.7110.7270.7190.5460.6250.5100.5050.5080.3350.3120.3610.3490.3550.1820.1560.2940.2860.2900.11700.1750.1700.173 | |||||||
货期：3-5 working days
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