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产品描述：人血小板膜糖蛋白Ⅳ(GP-Ⅳ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD36含量。CD36是一种跨膜糖蛋白，在多种细胞类型中表达，具有结合脂质、TSP1等配体的能力，并通过细胞内结构域与其他受体相互作用传递信号。其研究机制涉及脂肪酸吸收、免疫识别、炎症、分子粘附和凋亡等过程。试剂盒检测范围为2.5 ng/mL-160 ng/mL，适用于心血管疾病模型、血液系统研究或炎症相关机制探索等科研领域，可帮助研究者分析 GP - Ⅳ在不同生理病理状态下的动态变化及其生物学作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD36; GP3B; GP4; Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD antigen CD36
缩写：CD36
Uniprot No.：P16671
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 ng/mL-160 ng/mL
灵敏度：0.625 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GP-Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human GP-Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1602.7832.7772.7802.663802.1342.1172.1262.009401.4961.4781.4871.370200.8550.8370.8460.729100.4170.4040.4110.29450.3210.3340.3280.2112.50.2250.2160.2210.10400.1180.1160.117 | ||||||
货期：3-5 working days
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