首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD34分子酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD34含量。CD34是一种细胞表面糖蛋白，在造血干细胞、血管内皮祖细胞等表达。其背景在于对造血系统研究有重要意义。研究机制上，它可作为识别标记用于分离和富集造血干细胞，还参与细胞黏附、迁移等过程，助力造血调控的研究。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究中CD34蛋白水平的定量分析，如干细胞分化调控实验、血管生成模型评估、血液系统疾病机制探索等基础科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34
缩写：CD34
Uniprot No.：P28906
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 96-103 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 97-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human CD34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-96 | ||||
| EDTA plasma (n=4) | 96 | 93-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4412.4782.4602.353101.8761.9121.8941.78751.2441.2561.2501.1432.50.6890.7070.6980.5911.250.4240.4480.4360.3290.6250.3230.3110.3170.2100.3120.2020.1890.1960.08900.1090.1050.107 | ||||||
货期：3-5 working days
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