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产品描述：人髓细胞表面抗原CD33(CD33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD33含量。CD33是一种跨膜糖蛋白，主要表达于髓系细胞表面。其在急性髓系白血病等血液系统肿瘤细胞上也有表达。科研人员针对它研究开发靶向药物，通过特异性结合CD33，抑制肿瘤细胞生长、诱导凋亡，为相关疾病治疗带来新策略。试剂盒检测范围为125 pg/mL-8000 pg/mL，本试剂盒可用于白血病细胞模型的功能研究、造血系统疾病相关生物标志物探索、免疫调控机制分析等领域，还能为免疫学、肿瘤学及血液系统疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33
缩写：CD33
Uniprot No.：P20138
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-113 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human CD33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-104 | ||||
| EDTA plasma (n=4) | 95 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.1302.1742.1521.99340001.7321.7541.7431.58420001.3341.3551.3451.18610000.8260.8500.8380.6795000.5650.5800.5730.4142500.4200.4170.4190.2601250.3020.2950.2990.14000.1540.1640.159 | ||||||
货期：3-5 working days
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