首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人钙结合蛋白(CALB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CALB1含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经生物学、肿瘤微环境分析、钙信号通路调控等基础研究，特别适合用于探究CALB1在帕金森病、阿尔茨海默病模型或乳腺癌等实体瘤中的分子机制。通过，研究者可高效获取样本中低丰度CALB1的定量数据，为细胞功能研究和疾病模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：avian-type ELISA Kit; CAB27 ELISA Kit; CALB 1 ELISA Kit; CALB ELISA Kit; CALB1 ELISA Kit; CALB1_HUMAN ELISA Kit; Calbindin 1 28kDa ELISA Kit; Calbindin ELISA Kit; Calbindin D28 ELISA Kit; D 28K ELISA Kit; D-28K ELISA Kit; D28K ELISA Kit; OTTHUMP00000166027 ELISA Kit; OTTHUMP00000225441 ELISA Kit; RTVL H protein ELISA Kit; Vitamin D dependent calcium binding protein ELISA Kit; Vitamin D dependent calcium binding protein avian type ELISA Kit; Vitamin D-dependent calcium-binding protein ELISA Kit
缩写：CALB1
Uniprot No.：P05937
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human calbindin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human calbindin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 95-110 | ||||
| EDTA plasma (n=4) | 98 | 93-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1782.3022.2402.0395001.5741.5321.5531.3522501.0541.0761.0650.8641250.7640.7910.7780.57762.50.4780.4520.4650.26431.250.3510.3820.3670.16615.60.2660.2580.2620.06100.1970.2040.201 | ||||||
货期：3-5 working days
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