首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人碳酸酐酶2(CA-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CA2含量。CA2即碳酸酐酶2，是一种锌金属酶，广泛分布于人体组织细胞中，能可逆催化二氧化碳水合反应。研究机制上，它与调节酸碱平衡、离子转运等密切相关，在青光眼、癌症等疾病治疗中是潜在靶点，通过抑制其活性可发挥治疗作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒基于高亲和力的特异性抗体构建，适用于科研领域中对CA-2蛋白的定量分析，可支持细胞代谢研究、疾病机制探索或生物标志物筛选等实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CA 2 ELISA Kit; CA II ELISA Kit; CA-II ELISA Kit; Ca2 ELISA Kit; CAC ELISA Kit; CAH2_HUMAN ELISA Kit; CAII ELISA Kit; Car 2 ELISA Kit; Car2 ELISA Kit; Carbonate dehydratase II ELISA Kit; Carbonic anhydrase 2 ELISA Kit; Carbonic anhydrase B ELISA Kit; Carbonic anhydrase C ELISA Kit; Carbonic anhydrase C; formerly ELISA Kit; Carbonic anhydrase II ELISA Kit; Carbonic dehydratase ELISA Kit; epididymis luminal protein 76 ELISA Kit; Epididymis secretory protein Li 282 ELISA Kit; HEL-76 ELISA Kit; HEL-S-282 ELISA Kit
缩写：CA2
Uniprot No.：P00918
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of human CA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 91 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.7002.5422.6212.502 102.1912.1132.1522.033 51.3741.3511.3631.244 2.50.7650.7540.7600.641 1.250.3730.3790.3760.257 0.6250.3100.3230.3170.198 0.3120.2150.2110.2130.094 00.1200.1180.119 | ||||||
货期：3-5 working days
×
