首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人碳酸酐酶3(CA3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CA3含量。CA3 作为一种靶点，在神经科学等领域有重要研究价值。它是大脑海马体的重要组成部分，在学习、记忆等认知功能中起关键作用。研究机制主要围绕其神经环路、信号传导及相关分子机制，以深入了解其功能及在疾病中的变化。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于肌肉生物学研究、代谢性疾病模型分析、运动生理学评估等科研领域，为探索CA3在能量代谢、肌肉功能调控及相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CA III ELISA Kit; CA-III ELISA Kit; Ca3 ELISA Kit; CAH3_HUMAN ELISA Kit; CAIII ELISA Kit; Car3 ELISA Kit; Carbonate dehydratase III ELISA Kit; Carbonic anhydrase 3 ELISA Kit; Carbonic anhydrase III ELISA Kit; Carbonic anhydrase III muscle specific ELISA Kit; EC 4.2.1.1 ELISA Kit; Epididymis secretory sperm binding protein Li 167mP ELISA Kit; HEL S 167mP ELISA Kit
缩写：CA3
Uniprot No.：P07451
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-107 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of human CA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 5002.7482.5792.6642.512 2502.1872.1362.1622.010 1251.4671.4881.4781.326 62.50.8970.7220.8100.658 31.20.4880.4900.4890.337 15.60.3460.3450.3460.194 7.80.2250.2370.2310.079 00.1560.1480.152 | ||||||
货期：3-5 working days
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