首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人B1缓激肽受体(BDKRB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BDKRB1含量。BDKRB1 是一个重要靶点。它在体内生理病理过程里有重要作用。研究表明其参与炎症、疼痛等机制，激活后能引发一系列细胞信号传导反应，调节血管通透性等。围绕它的研究对开发治疗炎症疾病、疼痛等药物有重要意义。试剂盒检测范围为25 pg/mL-1600 pg/mL，可广泛应用于基础科研领域，例如探究炎症相关疾病模型中受体表达动态变化、评估药物干预对缓激肽信号通路的影响，或用于细胞信号转导机制研究，为相关疾病的分子机制解析及潜在治疗靶点筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BDKRB1; BRADYB1; B1 bradykinin receptor; B1R; BK-1 receptor
缩写：BDKRB1
Uniprot No.：P46663
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BDKRB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 99-107 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 96-108 | ||||||
回收率：
| The recovery of human BDKRB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 89-98 | |||||
| EDTA plasma (n=4) | 92 | 87-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16001.7741.8451.8101.6248001.3211.4281.3751.1894001.0150.9820.9990.8132000.7120.7010.7070.5211000.4530.4690.4610.275500.3110.3240.3180.132250.2420.2490.2460.06000.1850.1870.186 | |||||||
货期：3-5 working days
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