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产品描述：人BCL2样蛋白11(BCL2L11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BCL2L11含量。BCL2L11是重要靶点。它属于BCL-2蛋白家族，在调控细胞凋亡中起关键作用。研究发现其异常表达与肿瘤发生发展密切相关，通过调节线粒体凋亡途径来影响细胞命运，是开发抗癌药物等治疗方案的潜在重要靶点。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，该产品能有效支持肿瘤发生机制研究、神经退行性疾病模型中的凋亡路径分析、免疫细胞功能调控实验以及药物开发过程中凋亡相关生物标志物的动态监测，为体外培养体系或动物模型中的蛋白表达水平检测提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BCL2 like 11 ELISA Kit; B2L11_HUMAN ELISA Kit; BAM ELISA Kit; Bcl 2 interacting protein Bim ELISA Kit; Bcl 2 related ovarian death agonist ELISA Kit; Bcl-2-like protein 11 ELISA Kit; BCL2 interacting mediator of cell death ELISA Kit; BCL2 like 11 (apoptosis facilitator) ELISA Kit; BCL2 like protein 11 ELISA Kit; Bcl2-interacting mediator of cell death ELISA Kit; Bcl2-L-11 ELISA Kit; Bcl2l11 ELISA Kit; BIM alpha6 ELISA Kit; BIM ELISA Kit; BIM beta6 ELISA Kit; BIM beta7 ELISA Kit; BimEL ELISA Kit; BimL ELISA Kit; BOD ELISA Kit
缩写：BCL2L11
Uniprot No.：O43521
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BCL2L11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 84-97 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-105 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of human BCL2L11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 102 | 98-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.1942.1872.1911.9917501.5061.5371.5221.3223751.0121.0361.0240.824187.50.6590.6890.6740.47493.750.4450.4260.4360.23646.880.3450.3240.3350.13523.440.2600.2820.2710.07100.2010.1980.200 | ||||||
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