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产品描述：人B细胞淋巴瘤因子6(Bcl6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BCL6含量。BCL6是重要靶点。它在正常淋巴细胞发育中发挥调节作用，异常表达会导致肿瘤发生。研究机制上，聚焦其在肿瘤细胞增殖、存活及免疫逃逸中的作用，通过干预BCL6相关信号通路，有望为肿瘤治疗开辟新途径。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于科研领域中对B细胞发育调控、免疫微环境分析以及淋巴瘤病理机制的研究，例如通过检测不同处理条件下细胞模型、动物模型或临床前样本中Bcl6的动态变化，探究其在细胞分化、肿瘤发生中的分子作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B cell CLL/lymphoma 6 ELISA Kit; B cell lymphoma 6 protein ELISA Kit; B-cell lymphoma 5 protein ELISA Kit; B-cell lymphoma 6 protein ELISA Kit; BCL 5 ELISA Kit; Bcl 6 ELISA Kit; BCL-5 ELISA Kit; BCL-6 ELISA Kit; BCL5 ELISA Kit; BCL6 ELISA Kit; BCL6_HUMAN ELISA Kit; BCL6A ELISA Kit; cys his2 zinc finger transcription factor ELISA Kit; cys-his2 zinc finger transcription factor ELISA Kit; LAZ 3 ELISA Kit; LAZ 3 protein ELISA Kit; LAZ3 ELISA Kit; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) ELISA Kit; Lymphoma associated zinc finger gene on chromosome 3 ELISA Kit; Protein LAZ-3 ELISA Kit; ZBTB 27 ELISA Kit; ZBTB27 ELISA Kit; Zinc finger and BTB domain containing protein 27 ELISA Kit; Zinc finger and BTB domain-containing protein 27 (ZBTB27) ELISA Kit; Zinc finger and BTB domain-containing protein 27 ELISA Kit; Zinc Finger Protein 51 (ZNF51) ELISA Kit; Zinc finger protein 51 ELISA Kit; zinc finger transcription factor BCL6S ELISA Kit; ZNF 51 ELISA Kit; ZNF51 ELISA Kit
缩写：BCL6
Uniprot No.：P41182
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 97-104 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
回收率：
| The recovery of human Bcl6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 87 | 83-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected12002.7902.7012.7462.5886002.1582.0322.0951.9373001.2691.3041.2871.1291500.7840.8110.7980.640750.5160.5310.5240.36637.50.3420.3360.3390.18118.750.2460.2520.2490.09100.1570.1590.158 | |||||||
货期：3-5 working days
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