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产品描述：大鼠Bcl-2样蛋白1(Bcl2-L-1/Bcl-X)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BCL2L1含量。BCL2L1是重要的抗凋亡蛋白家族成员，其高表达会抑制细胞凋亡，与肿瘤发生、发展及耐药密切相关。研究聚焦于其抑制凋亡的分子机制，通过开发靶向抑制剂来促进癌细胞凋亡，为癌症治疗提供新策略，有广阔的潜在应用价值。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于体外培养细胞或动物模型中Bcl - X蛋白表达水平的动态分析，如药物干预下的凋亡通路研究、疾病模型中的蛋白功能验证或肿瘤治疗相关机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bcl2l1; Bclx; Blc2l; Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X
缩写：BCL2L1
Uniprot No.：P53563
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Bcl2-L-1/Bcl-X in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of rat Bcl2-L-1/Bcl-X spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 88-94 | ||||
| EDTA plasma (n=4) | 99 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.1062.0082.0571.92815001.4161.3761.3961.2677500.9280.9650.9470.8183750.5960.6050.6010.472187.50.3640.3530.3590.23093.750.2930.2840.2890.16046.880.2150.2070.2110.08200.1300.1280.129 | ||||||
货期：3-5 working days
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