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产品描述：人B细胞淋巴瘤-XL(Bcl-xl)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的Bcl-xl含量。Bcl-xl是一种抗凋亡蛋白，属于Bcl-2家族。其背景在于过度表达与肿瘤发生、发展及耐药性相关。研究机制上，它主要通过与促凋亡蛋白相互作用来抑制细胞凋亡，影响细胞存活，是肿瘤治疗等领域的重要潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于细胞凋亡机制研究、肿瘤生物学模型评估或药物干预实验中Bcl-xl蛋白动态变化的监测，为探索Bcl-xl在疾病进程中的作用及靶向治疗开发提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI325008 ELISA Kit; BAD ELISA Kit; BAD_HUMAN ELISA Kit; BBC 2 ELISA Kit; BBC2 ELISA Kit; BBC6 ELISA Kit; Bcl 2 Antagonist of Cell Death ELISA Kit; Bcl 2 Binding Component 6 ELISA Kit; BCL X / BCL 2 Binding Protein ELISA Kit; BCL X Binding Protein ELISA Kit; Bcl XL/Bcl 2 Associated Death Promoter ELISA Kit; Bcl-2-binding component 6 ELISA Kit; Bcl-2-like protein 8 ELISA Kit; Bcl-XL/Bcl-2-associated death promoter ELISA Kit; Bcl2 antagonist of cell death ELISA Kit; BCL2 antagonist of cell death protein ELISA Kit; BCL2 associated agonist of cell death ELISA Kit; Bcl2 Associated Death Promoter ELISA Kit; BCL2 binding component 6 ELISA Kit; BCL2 binding protein ELISA Kit; Bcl2 Like 8 Protein ELISA Kit; Bcl2-L-8 ELISA Kit; BCL2L8 ELISA Kit; Proapoptotic BH3 Only Protein ELISA Kit
缩写：Bcl-xl
Uniprot No.：Q07817
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl-xl in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human Bcl-xl spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.4152.3522.3842.190 51.9341.8761.9051.711 2.51.4051.5041.4551.261 1.250.9020.9390.9210.727 0.6250.5440.5240.5340.340 0.3120.3780.3590.3690.175 0.1560.2910.3090.3000.106 00.1910.1970.194 | ||||||
货期：3-5 working days
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