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产品描述：人精氨酸酶-1(ARG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、腹水样本中的ARG1含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于肝病模型研究、代谢性疾病机制探索、免疫细胞功能分析以及肿瘤微环境相关科研实验，为体外检测ARG1表达水平提供可靠工具。其优化的反应体系支持多种生物样本类型，配套标准品与预包被板确保实验稳定性和重复性，满足基础研究与药物开发中对精氨酸酶活性动态监测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A I ELISA Kit; Al ELISA Kit; ARG 1 ELISA Kit; arg1 ELISA Kit; ARGI1_HUMAN ELISA Kit; Arginase 1 ELISA Kit; Arginase liver ELISA Kit; Arginase type I ELISA Kit; Arginase; liver ELISA Kit; Arginase-1 ELISA Kit; Arginase1 ELISA Kit; Liver type arginase ELISA Kit; Liver-type arginase ELISA Kit; Type I arginase ELISA Kit
缩写：ARG1
Uniprot No.：P05089
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, ascitic fluid
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-93 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 100-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of human ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-102 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.2542.1542.2042.063501.6121.5121.5621.421251.0311.0111.0210.88012.50.5460.5360.5410.4006.250.3380.3280.3330.1923.120.2670.2570.2620.1211.560.1990.1980.1990.05800.1420.1400.141 | ||||||
货期：3-5 working days
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