首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞骨架活性调节蛋白(ARC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ARC含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，该产品专为科研场景设计，可应用于细胞骨架相关机制研究，如探究ARC在细胞迁移、神经可塑性或疾病模型（如神经退行性疾病、肿瘤转移）中的表达调控，也可用于药物干预或基因编辑后ARC水平变化的动态监测，为体外实验提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：activity regulated cytoskeleton associated protein ELISA Kit; activity regulated gene 3.1 protein homolog ELISA Kit; Activity-regulated cytoskeleton-associated protein ELISA Kit; Activity-regulated gene 3.1 protein homolog ELISA Kit; Arc ELISA Kit; ARC/ARG3.1 ELISA Kit; ARC_HUMAN ELISA Kit; Arg 3.1 ELISA Kit; Arg3.1 ELISA Kit
缩写：ARC
Uniprot No.：Q7LC44
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 101-113 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 102-109 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 92-98 | ||||||
回收率：
| The recovery of human ARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-103 | |||||
| EDTA plasma (n=4) | 88 | 86-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.8052.7202.7632.6528001.3961.5151.4561.3454000.7860.8420.8140.7032000.4810.4710.4760.3651000.2860.2750.2810.170500.1820.1860.1840.073250.1340.1320.1330.02200.1090.1120.111 | |||||||
货期：3-5 working days
×
