首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠角化细胞内分泌因子(KAF)/双调蛋白(AR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AREG含量。AREG（Amphiregulin）是一种表皮生长因子家族成员，主要在肿瘤组织中高表达，与肿瘤的增殖、侵袭和转移密切相关。研究显示，AREG基因的高表达与患者年龄及肿瘤细胞分化程度密切相关，其高表达可能与启动子区去甲基化有关。此外，AREG在多种恶性肿瘤中异常活跃，如结直肠癌、非小细胞肺癌等，其过表达会加速肿瘤生长和转移。试剂盒检测范围为1.25 pg/mL-80 pg/mL，该产品为科研领域提供可靠工具，可用于研究皮肤疾病模型、上皮损伤修复机制、慢性炎症反应或肿瘤微环境中KAF/AR的动态变化，特别适用于动物实验中的分子机制探索及生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
缩写：AREG
Uniprot No.：P24338
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.31 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 98-108 | ||||
| EDTA plasma (n=4) | 97 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected802.5382.6162.5772.413401.9392.0591.9991.835201.3641.3861.3751.211100.8820.8700.8760.71250.5490.5410.5450.3812.50.3410.3560.3490.1851.250.2530.2580.2560.09200.1680.1600.164 | ||||||
货期：3-5 working days
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