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产品描述：人角化细胞内分泌因子(KAF)/双调蛋白(AR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的AREG含量。AREG（Amphiregulin）是一种生长因子，属于表皮生长因子家族，参与调控多种生理过程，如细胞增殖、分化、迁移和凋亡。在肿瘤发生发展中，AREG表达上调，促进肿瘤细胞的生长、侵袭和转移。研究机制表明，AREG通过与其受体EGFR结合，激活下游信号通路，如ERK、AKT和STAT3，从而促进肿瘤的发生发展。靶向抑制AREG/EGFR信号轴可能成为治疗肿瘤的新策略。试剂盒检测范围为4.7 pg/mL-300 pg/mL，该产品可广泛应用于基础医学研究中皮肤疾病模型构建、创伤修复机制探索、炎症因子网络解析以及肿瘤生物学相关实验，为体外检测生物样本中KAF/AR的动态变化提供标准化工具，实验流程简便且稳定性高，适用于细胞培养上清或体液样本的批量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AREG; AREGB; SDGF; Amphiregulin; AR; Colorectum cell-derived growth factor; CRDGF
缩写：AREG
Uniprot No.：P15514
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.1 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 95-101 | |||||
回收率：
| The recovery of human KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.8122.8362.8242.6811501.9711.9651.9681.825751.1221.1681.1451.00237.50.5900.5710.5810.43818.80.3850.3990.3920.2499.40.2930.2730.2830.1404.70.2100.2160.2130.07000.1420.1440.143 | ||||||
货期：3-5 working days
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