首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠雄激素受体(AR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AR含量。AR（雄激素受体）是一种核受体。其在雄激素信号通路中起关键作用，与前列腺癌等疾病发展密切相关。研究机制主要聚焦于探究其活性调节、与配体相互作用等，以寻找抑制其功能的方法，为相关疾病治疗提供思路。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于研究雄激素相关生理调控机制，如生殖内分泌研究、代谢性疾病模型分析、肌肉组织功能评估等领域，也可用于药物开发过程中对雄激素通路干预效果的体外检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ar ELISA Kit; Nr3c4Androgen receptor ELISA Kit; Dihydrotestosterone receptor ELISA Kit; Nuclear receptor subfamily 3 group C member 4 ELISA Kit
缩写：AR
Uniprot No.：P15207
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 106 | |||||
| Range % | 98-111 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 95-107 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 88-102 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 98-109 | ||||||
回收率：
| The recovery of rat AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 94-109 | |||||
| EDTA plasma (n=4) | 97 | 87-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.1982.1052.1522.0427501.8441.8031.8241.7143751.4261.3761.4011.291187.51.1201.0871.1040.994940.6500.6410.6460.536470.4700.4560.4630.35323.50.3150.3070.3110.20100.1080.1120.110 | |||||||
货期：3-5 working days
×
