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产品描述：人水通道蛋白4(AQP-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AQP4含量。AQP4是一种水通道蛋白，主要分布于中枢神经系统。其与脑脊液生成、平衡及脑水肿等病理过程密切相关。研究机制方面，聚焦于它在细胞水转运中的作用，以及其表达异常与神经系统疾病如脑损伤、多发性硬化症等的关联。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于神经科学、免疫学及疾病机制研究领域，可应用于脑组织、脊髓或体液样本中AQP - 4表达水平的动态监测，为探索神经炎症、水代谢失衡等病理模型提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AQP 4 ELISA Kit; AQP-4 ELISA Kit; AQP4 ELISA Kit; AQP4_HUMAN ELISA Kit; Aquaporin type 4 ELISA Kit; Aquaporin-4 ELISA Kit; Aquaporin4 ELISA Kit; HMIWC 2 ELISA Kit; HMIWC2 ELISA Kit; Mercurial insensitive water channel ELISA Kit; Mercurial-insensitive water channel ELISA Kit; MGC22454 ELISA Kit; MIWC ELISA Kit; WCH 4 ELISA Kit; WCH4 ELISA Kit
缩写：AQP4
Uniprot No.：P55087
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 88-93 | |||||
回收率：
| The recovery of human AQP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 90-106 | ||||
| EDTA plasma (n=4) | 92 | 85-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5682.5552.5622.47251.7251.6781.7021.6122.51.1131.1011.1071.0171.250.6470.6390.6430.5530.6250.3810.3560.3690.2790.3120.2180.2090.2140.1240.1560.1470.1370.1420.05200.0920.0880.090 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human AQP-4 antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AQP-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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