首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人水通道蛋白3(AQP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AQP3含量。AQP3（水通道蛋白3）是一种疏水性膜转运蛋白，主要参与水及甘油的运输。其在消化系中发挥重要作用，与紧密连接相关蛋白Occludin和Claudin-1的表达密切相关，影响肠黏膜屏障功能。研究显示，AQP3在肿瘤血管生成和进展中起重要作用，可能成为治疗肿瘤的新靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒支持科研人员高效开展与AQP-3相关的机制研究，例如皮肤疾病模型中水合作用变化分析、代谢相关疾病中甘油转运功能探索，或肿瘤微环境渗透压调控机制等基础研究领域。试剂盒兼容多种生物样本类型，可为体外实验提供稳定可靠的定量检测工具，适用于细胞生物学、分子病理学及生理学等科研场景的数据采集需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AQP3; Aquaporin-3; AQP-3; Aquaglyceroporin-3
缩写：AQP3
Uniprot No.：Q92482
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:10 | Average % | 90 | ||||
| Range % | 85-100 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of human AQP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-105 | ||||
| EDTA plasma (n=4) | 95 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1232.1312.1271.994101.7741.7921.7831.65051.1561.1631.1601.0272.50.5420.5490.5460.4131.250.3510.3480.3500.2170.6250.2300.2410.2360.1030.3120.1790.1830.1810.04800.1320.1340.133 | ||||||
货期：3-5 working days
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