| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | E4; UFD2; UBOX2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human UBE4A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE4A抗体的虚构参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：UBE4A Antibody Characterization in Neurodegenerative Disease Models
**作者**：Chen L, et al.
**摘要**：本研究利用UBE4A特异性抗体，通过免疫印迹和免疫组化分析其在阿尔茨海默病小鼠脑组织中的表达变化，发现UBE4A与淀粉样蛋白沉积呈负相关，提示其可能参与蛋白质稳态调控。
2. **文献名称**：UBE4A Interaction with p53: A Role in Cancer Progression
**作者**：Smith JR, et al.
**摘要**：通过免疫共沉淀(Co-IP)和UBE4A抗体验证，揭示了UBE4A与肿瘤抑制因子p53的相互作用，表明其在泛素化介导的p53降解中发挥作用，可能影响结直肠癌的化疗耐药性。
3. **文献名称**：Development of a High-Affinity Monoclonal Antibody for UBE4A Functional Studies
**作者**：Wang Y, et al.
**摘要**：报道了一种新型UBE4A单克隆抗体的开发，验证其在流式细胞术和免疫荧光中的特异性，并应用于筛选UBE4A在多种肿瘤细胞系中的亚细胞定位差异。
4. **文献名称**：UBE4A Regulates Autophagy via mTOR Pathway: Evidence from Knockdown and Antibody-Based Assays
**作者**：Garcia M, et al.
**摘要**：结合siRNA敲低和UBE4A抗体检测，证明UBE4A通过调控mTOR信号通路影响自噬活性，为神经退行性疾病和代谢紊乱提供了潜在机制解释。
注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。
The UBE4A (Ubiquitination Factor E4A) antibody is a crucial tool for studying the UBE4A protein, a member of the UBE4 family of ubiquitin ligases involved in the ubiquitin-proteasome system (UPS). UBE4A plays a role in substrate recognition and degradation by catalyzing the formation of polyubiquitin chains, marking proteins for proteasomal breakdown. It is implicated in cellular processes like DNA repair, apoptosis, and neuronal development, with dysregulation linked to neurodegenerative disorders and cancers.
UBE4A antibodies are widely used in research applications such as Western blotting, immunoprecipitation, and immunohistochemistry (IHC) to detect protein expression, localization, and interaction partners. These antibodies are essential for exploring UBE4A's regulatory mechanisms, including its role in modulating pathways like the p53 signaling network or stress responses. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high specificity, aiding in distinguishing UBE4A from homologous family members (e.g., UBE4B).
Validated UBE4A antibodies are critical for ensuring experimental reproducibility. Commercial antibodies are typically raised in hosts like rabbits or mice, with validation using knockout cell lines or siRNA knockdown to confirm target specificity. Researchers prioritize antibodies with demonstrated cross-reactivity data across species (human, mouse, rat) and compatibility with specific experimental conditions. Reliable UBE4A antibodies advance understanding of its pathophysiological roles and potential therapeutic targeting in diseases.
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