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产品描述：人水通道蛋白1(AQP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的AQP1含量。AQP1（水通道蛋白1）是一种跨膜蛋白，主要介导水分子的跨膜转运，并参与细胞迁移、增殖和血管生成等过程。研究表明，AQP1在肿瘤进展中发挥重要作用，其表达上调可促进肿瘤细胞迁移、增殖和血管生成，可能成为肿瘤治疗的潜在靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，支持科研人员开展细胞水转运机制、病理生理模型构建或体外药物干预实验等基础研究；为探索AQP - 1在体液代谢异常、组织水肿或肿瘤微环境重塑等领域的生物学功能提供可靠工具；适用于分子生物学实验室常规检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AQP1; CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
缩写：AQP1
Uniprot No.：P29972
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 90-103 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human AQP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-95 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0702.0552.0631.9555001.6501.5921.6211.5132501.2861.2781.2821.1741250.8870.9250.9060.79862.50.5210.5630.5420.43431.20.3620.3450.3540.24615.60.2450.2350.2400.13200.1120.1030.108 | ||||||
货期：3-5 working days
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