首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白O(APOO)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOO含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于基础科研中APOO在肝脏、脂肪组织等代谢相关器官的表达分析，也可用于体外实验模型（如细胞培养上清）或动物实验中脂代谢通路的功能研究，为探索脂质稳态调控、能量代谢疾病机制及潜在干预策略提供可靠工具，严格限用于生命科学领域研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOO; FAM121B; MIC23; MIC26; My025; UNQ1866/PRO4302; MICOS complex subunit MIC26; Apolipoprotein O; MICOS complex subunit MIC23; Protein FAM121B
缩写：APOO
Uniprot No.：Q9BUR5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.08 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 102 | |||||
| Range % | 97-106 | ||||||
| 1:200 | Average % | 86 | |||||
| Range % | 83-91 | ||||||
| 1:400 | Average % | 108 | |||||
| Range % | 104-112 | ||||||
| 1:800 | Average % | 85 | |||||
| Range % | 81-90 | ||||||
回收率：
| The recovery of human APOO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 97-106 | |||||
| EDTA plasma (n=4) | 89 | 85-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 200.1220.1260.124 100.2070.2150.211 50.3260.3310.329 2.50.5530.5490.551 1.250.8460.8540.850 0.6251.1971.2001.199 0.3121.5861.5981.592 02.3872.4002.394 | |||||||
货期：3-5 working days
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