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产品描述：山羊脂联素(ADP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADP含量。ADP即二磷酸腺苷，是生物体内能量代谢的关键分子。在细胞代谢中，它参与能量的储存和释放。研究机制方面，ADP可与相关受体结合，调节血小板聚集等生理过程，还在信号传导等通路中发挥作用，成为药物研发等领域的重要靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒适用于肥胖、2型糖尿病、心血管疾病等代谢相关机制的基础研究，可支持动物模型或体外实验中脂联素表达水平的动态监测，为探索能量代谢调控、脂肪因子互作等科研方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ADP
Uniprot No.：U6A087
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
| 1:2000 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
| 1:4000 | Average % | 99 | |||||
| Range % | 93-104 | ||||||
| 1:8000 | Average % | 104 | |||||
| Range % | 97-109 | ||||||
回收率：
| The recovery of goat ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-97 | |||||
| EDTA plasma (n=4) | 94 | 87-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 200.1920.1900.191 100.4060.3830.395 50.6920.6560.674 2.51.0100.9740.992 1.251.4261.3891.408 0.6251.9301.8721.901 0.3122.2322.3152.274 02.4632.4002.432 | |||||||
货期：3-5 working days
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