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产品描述：人A解聚素和金属蛋白酶及血小板反应蛋白基序9(ADAMTS9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ADAMTS9含量。ADAMTS9是一种具有血小板反应蛋白基序的ADAM金属肽酶，编码ADAMTS蛋白家族成员。该家族成员共享多个蛋白质模块，包括前肽区域、金属蛋白酶结构域等，参与蛋白聚糖裂解、器官形状控制及血管生成抑制。研究机制涉及ADAMTS9与蛋白聚糖、细胞外基质等相互作用，以及其在多种疾病如遗传性肾肿瘤、早产胎膜早破等中的作用。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于体外培养细胞模型中蛋白酶分泌机制研究、疾病动物模型组织内源性ADAMTS9水平检测，以及通过临床前样本（如血清、血浆）探索其与特定病理过程的相关性，为探究ADAMTS9在组织修复、血管生成及代谢调控中的分子机制提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAMTS9 ELISA Kit; KIAA1312 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 9 ELISA Kit; ADAM-TS 9 ELISA Kit; ADAM-TS9 ELISA Kit; ADAMTS-9 ELISA Kit; EC 3.4.24.- ELISA Kit
缩写：ADAMTS9
Uniprot No.：Q9P2N4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human ADAMTS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-106 | ||||
| EDTA plasma (n=4) | 87 | 94-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.5002.4882.4942.398251.4581.3761.4171.32112.50.7170.6840.7010.6056.250.3580.3390.3490.2533.120.2270.2140.2210.1251.560.1610.1590.1600.0640.780.1260.1180.1220.02600.0970.0950.096 | ||||||
货期：3-5 working days
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