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Human ADAM metallopeptidase with thrombospondin type 1 motif4,ADAMTS4 ELISA Kit

  • 中文名： 人ADAM金属肽酶含血小板反应蛋白1基元4(ADAMTS4)ELISA Kit
货号: IPD-E11848h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人ADAM金属肽酶含血小板反应蛋白1基元4(ADAMTS4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS4含量。ADAMTS4 属于去整合素样金属蛋白酶家族，在降解软骨蛋白聚糖中起关键作用。其过度表达与骨关节炎等疾病密切相关。研究多围绕抑制其活性以延缓关节软骨退变展开，有望为骨关节炎等疾病的治疗提供新策略。试剂盒检测范围为12.5 ng/ml- 800 ng/ml，适用于基础研究中探索ADAMTS4在炎症性疾病、心血管病变及肿瘤微环境中的作用机制，也可用于评估实验性药物对基质代谢相关通路的影响。样本类型兼容性广泛，支持从体液到组织样本的多维度研究，为科研人员提供稳定可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    A disintegrin and metalloproteinase with thrombospondin motifs 4 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 ELISA Kit; A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 4 ELISA Kit; ADAM TS 4 ELISA Kit; ADAM TS4 ELISA Kit; ADAM-TS 4 ELISA Kit; ADAM-TS4 ELISA Kit; ADAMTS 2 ELISA Kit; ADAMTS 4 ELISA Kit; ADAMTS-4 ELISA Kit; ADAMTS2 ELISA Kit; ADAMTS4 ELISA Kit; ADAMTS4 protein ELISA Kit; ADMP 1 ELISA Kit; ADMP-1 ELISA Kit; ADMP1 ELISA Kit; Aggrecanase 1 ELISA Kit; Aggrecanase-1 ELISA Kit; Aggrecanase1 ELISA Kit; ATS4_HUMAN ELISA Kit; KIAA0688 ELISA Kit
  • 缩写：
    ADAMTS4
  • Uniprot No.：
    O75173
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/ml - 800 ng/ml
  • 灵敏度：
    3.125 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-92


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    96


    Range %

    92-100


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human ADAMTS4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    90-101


    EDTA plasma (n=4)

    98

    90-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    800

    2.748

    2.801

    2.775

    2.685


    400

    2.235

    2.241

    2.238

    2.148


    200

    1.542

    1.567

    1.555

    1.465


    100

    0.906

    0.943

    0.925

    0.835


    50

    0.446

    0.467

    0.457

    0.367


    25

    0.243

    0.264

    0.254

    0.164


    12.5

    0.152

    0.149

    0.151

    0.061


    0

    0.092

    0.087

    0.090




  • 货期：
    3-5 working days

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