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产品描述：小鼠金属肽酶含血小板反应蛋白5(ADAMTS5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS5含量。ADAMTS5 是重要的药物靶点。它属去整合素样金属蛋白酶家族，与软骨破坏密切相关，在骨关节炎等疾病发病机制中发挥关键作用。研究聚焦其在疾病进程中的作用机制，期望通过抑制它的活性来开发治疗骨关节炎等病症的药物。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对小鼠ADAMTS5表达调控的机制研究，如关节炎模型中的基质代谢分析、炎症相关信号通路探索或药物干预效果评估。支持科研人员在体外实验中系统量化ADAMTS5在生理或病理状态下的动态变化，为疾病机理研究及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adamts5 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit; ADAM-TS 5 ELISA Kit; ADAM-TS5 ELISA Kit; ADAMTS-5 ELISA Kit; EC 3.4.24.- ELISA Kit; ADMP-2 ELISA Kit; Aggrecanase-2 ELISA Kit; Implantin ELISA Kit
缩写：ADAMTS5
Uniprot No.：Q9R001
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 81-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 92-107 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-106 | |||||
回收率：
| The recovery of mouse ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-98 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.4232.4142.4192.236201.6211.5571.5891.406100.9040.9250.9150.73250.5430.5580.5510.3682.50.3820.3720.3770.1941.250.2990.3050.3020.1190.6250.2470.2560.2520.06900.1810.1850.183 | ||||||
货期：3-5 working days
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