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产品描述：人金属肽酶含血小板反应蛋白5(ADAMTS5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS5含量。ADAMTS5 即含血小板反应蛋白基序的解聚蛋白样金属蛋白酶 5，在软骨破坏中起重要作用。研究机制上，它可降解软骨中蛋白聚糖，引发关节软骨损伤，是骨关节炎治疗重要潜在靶点，针对它研发药物或疗法有望改善疾病进程。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，本试剂盒适用于基础研究中对ADAMTS5蛋白表达水平的动态监测，如关节软骨代谢研究、细胞外基质重塑机制探索或炎症相关信号通路分析等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A disintegrin and metalloproteinase with thrombospondin motifs 11 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) ELISA Kit; A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 5 ELISA Kit; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 5 ELISA Kit; ADAM TS 11 ELISA Kit; ADAM TS 5 ELISA Kit; ADAM TS5 ELISA Kit; ADAM-TS 11 ELISA Kit; ADAM-TS 5 ELISA Kit; ADAM-TS5 ELISA Kit; ADAMTS 11 ELISA Kit; ADAMTS 5 ELISA Kit; ADAMTS-11 ELISA Kit; ADAMTS-5 ELISA Kit; ADAMTS11 ELISA Kit; ADAMTS11, formerly ELISA Kit; Adamts5 ELISA Kit; ADMP 2 ELISA Kit; ADMP-2 ELISA Kit; ADMP2 ELISA Kit; Aggrecanase 2 ELISA Kit; Aggrecanase-2 ELISA Kit; ATS5_HUMAN ELISA Kit; FLJ36738 ELISA Kit; Implantin ELISA Kit; ThromboSpondin motif-5 ELISA Kit
缩写：ADAMTS5
Uniprot No.：Q9UNA0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-94 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of human ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-99 | ||||
| EDTA plasma (n=4) | 89 | 82-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.1542.2542.2042.062 5001.5121.6121.5621.420 2501.0011.0111.0060.864 1250.5360.5460.5410.399 62.50.3280.3280.3280.186 31.250.2570.2670.2620.120 15.60.1890.1990.1940.052 00.1420.1410.142 | ||||||
货期：3-5 working days
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