首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人促肾上腺皮质激素(ACTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTH含量。ACTH即促肾上腺皮质激素，由腺垂体分泌。其背景在于它可促进肾上腺皮质增生、合成与分泌肾上腺皮质激素。研究机制方面，它与肾上腺皮质细胞膜上受体结合，激活腺苷酸环化酶-cAMP-蛋白激酶系统，从而调节肾上腺皮质激素合成与分泌。试剂盒检测范围为12.5 pg/mL-200 pg/mL，适用于科研领域中对下丘脑 - 垂体 - 肾上腺轴功能的机制研究、疾病模型构建以及药物干预效果的评估；为研究ACTH在生理病理过程中的动态变化提供可靠工具，尤其适用于神经内分泌学、代谢性疾病及应激反应相关的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-200 pg/mL
灵敏度：12.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1920.1870.190 1000.3370.3280.333 500.6010.6130.607 251.1271.1401.134 12.52.3162.2122.264 | ||||||
货期：3-5 working days
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