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产品描述：人2,3-二磷酸甘油酸(2,3-DPG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的2,3DPG含量。2,3-DPG即2,3-二磷酸甘油酸，是红细胞糖酵解支路的中间产物。它能调节血红蛋白对氧气的亲和力，降低其与氧的结合力，促进氧气释放。在贫血、高原适应等过程中发挥重要作用。当前对其在相关疾病机制及治疗靶点方面有一定研究。试剂盒检测范围为0.312 μmol/mL-20 μmol/mL，适用于基础研究中红细胞代谢功能评估、缺氧生理机制探索以及血液系统疾病模型的机制分析，例如贫血、高原反应或心血管相关疾病的体外实验研究，满足细胞能量代谢、运动生理学或病理状态下的氧运输调控等科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：2,3DPG
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 μmol/mL-20 μmol/mL
灵敏度：0.078 μmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 96 | |
| Range % | 92-100 | ||
| 1:2 | Average % | 98 | |
| Range % | 92-103 | ||
| 1:4 | Average % | 95 | |
| Range % | 90-99 | ||
| 1:8 | Average % | 90 | |
| Range % | 85-96 |
回收率：
| The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µmol/mlOD1OD2AverageCorrected 202.8732.7122.7932.636 102.2652.1492.2072.050 51.3721.3281.3501.193 2.50.8540.8130.8340.677 1.250.4570.4810.4690.312 0.6250.3370.3570.3470.190 0.3120.2020.2070.2050.048 00.1610.1520.157 | ||||||
货期：3-5 working days
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