Mouse Tumor necrosis factor α,TNF-α ELISA KIT

产品详情

中文名： 小鼠肿瘤坏死因子α(TNF-α)ELISA KIT

货号: IPD-E04741m(1)

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：
小鼠肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测组织培养上清液、组织匀浆样本中的TNF含量。TNF 即肿瘤坏死因子，是一种细胞因子，在人体免疫反应、炎症调节等方面起重要作用。其背景与机体防御、免疫监视相关。研究机制上，它能与相应受体结合，激活多条信号通路，参与细胞存活、凋亡、炎症因子释放等过程，在多种疾病病理中受关注。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。适用于体外实验中细胞因子分泌水平检测、炎症模型评估、抗体药物效价分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写：
TNF
Uniprot No.：
P06804
种属：
Mus musculus (Mouse)
样本类型：
cell culture supernates, tissue homogenates
检测范围：
62.5 pg/mL-4000 pg/mL
灵敏度：
15.6 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-92
1:2	Average %	94
	Range %	91-97
1:4	Average %	99
	Range %	92-110
1:8	Average %	96
	Range %	89-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	2.727	2.634	2.680	2.509
2000	2.027	1.967	1.997	1.826
1000	1.236	1.158	1.197	1.026
500	0.742	0.729	0.735	0.564
250	0.428	0.421	0.424	0.253
125	0.366	0.353	0.359	0.188
62.5	0.268	0.274	0.271	0.100
0	0.167	0.175	0.171