Mouse Fibroblast growth factor 15(FGF15) ELISA kit

大包装询价

货号: IPD-EL522052MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠成纤维细胞生长因子15(FGF15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FGF15含量。FGF15，全称成纤维细胞生长因子15，是一种肠道激素，参与调节胆汁酸、葡萄糖和脂质代谢等，维持全身稳态。研究显示，FGF15通过血液作用于多个组织器官，调控肠肝轴和系统代谢，其失调可能促进多种疾病。研究机制方面，FGF15在肝脏中抑制胆汁酸生物合成，并通过表观遗传机制调控脂肪生成。试剂盒检测范围为15.6 pg/ml- 1000 pg/ml，灵敏度为3.9 pg/ml。适用于体外研究FGF15在代谢性疾病模型、肠道损伤修复、肝脏再生调控以及神经系统发育等实验中的表达变化，可为探索FGF15信号通路机制及靶向干预研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
FGF-15 ELISA Kit; Fgf15 ELISA Kit; FGF15_MOUSE ELISA Kit; FGF19 ELISA Kit; Fibroblast growth factor 15 ELISA Kit
缩写：
FGF15
Uniprot No.：
O35622
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates, cell lysates
检测范围：
15.6 pg/ml - 1000 pg/ml
灵敏度：
3.9 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	90
	Range %	85-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	87-97

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.754	2.622	2.688	2.580
500	2.215	2.185	2.200	2.092
250	1.534	1.496	1.515	1.407
125	0.699	0.647	0.673	0.565
62.5	0.366	0.358	0.362	0.254
31.2	0.265	0.249	0.257	0.149
15.6	0.187	0.179	0.183	0.075
0	0.112	0.103	0.108

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human FGF15 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled FGF15 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.