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Human Interferon γ (IFN-γ) ELISA Kit

  • 中文名： 人γ干扰素(IFN-γ)ELISA Kit
货号: IPD-E04577h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测组织培养上清液样本中的IFNG含量。IFNG，即干扰素-γ，是一种重要的II型干扰素，具有抗病毒、抗肿瘤和免疫调节功能。其研究机制主要涉及激活JAK/STAT信号通路，调节基因转录，影响免疫细胞活性，并在肿瘤微环境中发挥复杂的作用，既可促进肿瘤细胞凋亡，也可能促进肿瘤生长。试剂盒检测范围为0.312 ng/ml-20 ng/ml，灵敏度为0.078 ng/ml。该产品广泛应用于免疫调控机制研究、疫苗或生物制剂效价评估、感染性疾病模型建立等科研场景，特别适合用于检测T淋巴细胞、NK细胞等免疫细胞在体外刺激后分泌的IFN - γ水平，为炎症反应研究、药物作用靶点验证及细胞因子网络调控分析提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    IF 1 ELISA Kit; IFG ELISA Kit; IFI ELISA Kit; IFN gamma ELISA Kit; IFN immune ELISA Kit; IFN, immune ELISA Kit; IFN-gamma ELISA Kit; IFNG ELISA Kit; IFNG_HUMAN ELISA Kit; Immune interferon ELISA Kit; Interferon gamma ELISA Kit; Type II Interferon ELISA Kit
  • 缩写：
    IFNG
  • Uniprot No.：
    P01579
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    Cell Culture Supernates
  • 检测范围：
    0.312 ng/ml-20 ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Cell culture supernates(n=4)


    1:1

    Average %

    95


    Range %

    89-99


    1:2

    Average %

    91


    Range %

    87-96


    1:4

    Average %

    90


    Range %

    85-95


    1:8

    Average %

    94


    Range %

    89-102


  • 回收率：

    The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Cell culture supernates (n=5)

    97

    92-104


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.488

    2.569

    2.529

    2.368


    10

    1.721

    1.785

    1.753

    1.592


    5

    1.205

    1.253

    1.229

    1.068


    2.5

    0.789

    0.809

    0.799

    0.638


    1.25

    0.472

    0.487

    0.480

    0.319


    0.625

    0.361

    0.352

    0.357

    0.196


    0.312

    0.263

    0.257

    0.260

    0.099


    0

    0.164

    0.158

    0.161




  • 货期：
    3-5 working days

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