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Human Interleukin 12,IL-12/P70 ELISA KIT

  • 中文名: 人白介素12(IL-12/P70)酶联免疫试剂盒
货号: IPD-E04599h(1)
Price: ¥2500
数量:
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  • 产品描述:
    人白介素12(IL-12/P70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液样本中的IL-12/P70含量。IL-12/P70作为重要靶点,在免疫调节中扮演关键角色。它是一种细胞因子,能激活T细胞和NK细胞,增强机体免疫反应。相关研究旨在通过调控其表达或阻断其信号传导通路,用于治疗炎症性疾病、肿瘤等,为开发新疗法提供方向。试剂盒检测范围为31.25 pg/ml- 2000pg/ml,灵敏度为7.8 pg/ml。适用于免疫调节机制研究、炎症性疾病动物模型分析、肿瘤微环境中细胞因子表达监测等科研领域,特别在评估病原体感染、自身免疫疾病或药物干预后IL-12/P70动态变化的研究中具有重要应用价值,为探索Th1型免疫反应调控及免疫治疗策略开发提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    IL-12/P70
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, cell culture supernates
  • 检测范围:
    31.25 pg/ml - 2000pg/ml
  • 灵敏度:
    7.8 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 本试剂盒所含材料:


    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料:


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期:
        3-5 working days

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