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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 89 | ||||
Range % | 86-93 | |||||
1:2 | Average % | 100 | ||||
Range % | 96-104 | |||||
1:4 | Average % | 90 | ||||
Range % | 85-94 | |||||
1:8 | Average % | 99 | ||||
Range % | 96-102 |
The recovery of rat ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 91-98 | ||||
EDTA plasma (n=4) | 98 | 95-102 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/ml | OD1 | OD2 | Average | Corrected | ||
15 | 2.348 | 2.362 | 2.355 | 2.182 | ||
7.5 | 1.844 | 1.871 | 1.858 | 1.685 | ||
3.75 | 1.270 | 1.285 | 1.278 | 1.105 | ||
1.88 | 0.686 | 0.672 | 0.679 | 0.506 | ||
0.94 | 0.400 | 0.388 | 0.394 | 0.221 | ||
0.47 | 0.337 | 0.322 | 0.330 | 0.157 | ||
0.235 | 0.248 | 0.233 | 0.241 | 0.068 | ||
0 | 0.174 | 0.172 | 0.173 |
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