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Rat arginine vasopressin (AVP) ELISA kit

  • 中文名: 大鼠抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)ELISA Kit
货号: IPD-E12684r
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    大鼠抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的AVP含量。AVP 即精氨酸血管加压素,它在人体的水平衡、血压调节等方面起着重要作用。其研究机制主要围绕其与受体结合展开,通过作用于血管、肾脏等部位的受体,调节血管收缩、水重吸收等生理过程,目前相关研究在探索疾病治疗方面有一定价值。试剂盒检测范围为0.235 ng/ml-15 ng/ml.,灵敏度为0.05 ng/ml.。适用于科研领域探究内分泌调节机制、肾脏功能研究、高血压或水代谢异常动物模型的建立与验证,以及药物干预对体液平衡影响的实验室评估。为神经内分泌研究、代谢性疾病基础研究及药理学实验提供灵敏且稳定的检测方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    AvpVasopressin-neurophysin 2-copeptin ELISA Kit; AVP-NPII) [Cleaved into: Arg-vasopressin ELISA Kit; Arginine-vasopressin); Neurophysin 2 ELISA Kit; Neurophysin-I); Copeptin] ELISA Kit
  • 缩写:
    AVP
  • Uniprot No.:
    P01186
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.235 ng/ml-15 ng/ml.
  • 灵敏度:
    0.05 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    86-93


    1:2

    Average %

    100


    Range %

    96-104


    1:4

    Average %

    90


    Range %

    85-94


    1:8

    Average %

    99


    Range %

    96-102


  • 回收率:

    The recovery of rat ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    91-98


    EDTA plasma (n=4)

    98

    95-102


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    15

    2.348

    2.362

    2.355

    2.182


    7.5

    1.844

    1.871

    1.858

    1.685


    3.75

    1.270

    1.285

    1.278

    1.105


    1.88

    0.686

    0.672

    0.679

    0.506


    0.94

    0.400

    0.388

    0.394

    0.221


    0.47

    0.337

    0.322

    0.330

    0.157


    0.235

    0.248

    0.233

    0.241

    0.068


    0

    0.174

    0.172

    0.173




  • 货期:
    3-5 working days

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