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Human galanin,GAL ELISA Kit

  • 中文名： 人甘丙肽/甘丙素(GAL)ELISA Kit
货号: IPD-E09025h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人甘丙肽/甘丙素(GAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的GAL含量。GAL是一类糖结合蛋白，广泛存在于多种生物体内，参与细胞间的相互作用和信号传导。其研究机制主要涉及GAL与细胞表面糖基的识别和结合，进而影响细胞生长、分化、迁移和凋亡等生理过程。GAL在肿瘤、免疫和生殖等领域的疾病发生发展中扮演重要角色。试剂盒检测范围为62.5 pg/ml-4000 pg/ml.，灵敏度为15.6 pg/ml。该产品适用于神经生物学、内分泌学及代谢性疾病研究领域，可用于探索GAL在神经系统疾病动物模型中的表达变化、评估药物干预对GAL信号通路的影响，或分析临床前研究中组织样本的GAL水平动态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    GAL 1 ELISA Kit; GAL ELISA Kit; GAL GMAP ELISA Kit; GAL1 ELISA Kit; GALA_HUMAN ELISA Kit; Galanin message associated peptide ELISA Kit; Galanin message-associated peptide ELISA Kit; Galanin precursor ELISA Kit; Galanin prepropeptide ELISA Kit; Galanin related peptide ELISA Kit; Galanin/GMAP prepropeptide ELISA Kit; GALN ELISA Kit; GLNN ELISA Kit; GMAP ELISA Kit; MGC40167 ELISA Kit
  • 缩写：
    GAL
  • Uniprot No.：
    P22466
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    62.5 pg/ml-4000 pg/ml.
  • 灵敏度：
    15.6 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    85


    Range %

    81-89


    1:2

    Average %

    99


    Range %

    95-103


    1:4

    Average %

    100


    Range %

    98-102


    1:8

    Average %

    95


    Range %

    90-98


  • 回收率：

    The recovery of human GAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-97


    EDTA plasma (n=4)

    95

    90-98


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    4000

    2.618

    2.556

    2.587

    2.478


    2000

    2.054

    2.033

    2.044

    1.935


    1000

    1.237

    1.212

    1.225

    1.116


    500

    0.659

    0.631

    0.645

    0.536


    250

    0.413

    0.398

    0.406

    0.297


    125

    0.241

    0.224

    0.233

    0.124


    62.5

    0.175

    0.163

    0.169

    0.060


    0

    0.111

    0.107

    0.109




  • 货期：
    3-5 working days

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