首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰岛素样生长因子结合蛋白2(IGFBP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGFBP2含量。IGFBP2是一种结合蛋白，在调节胰岛素样生长因子活性方面发挥关键作用。其过表达与多种肿瘤的发生发展、侵袭转移及预后不良相关。研究聚焦于其在肿瘤微环境中的作用机制，探索以IGFBP2为靶点开发新型抗癌疗法，有望改善癌症治疗效果。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。可广泛应用于分子机制研究、疾病模型分析或生物标志物探索等科研领域，为探究 IGFBP2 在疾病发生发展中的作用提供可靠工具，特别适用于需要高灵敏度检测低丰度样本的研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BP 2 ELISA Kit; BP2 ELISA Kit; IBP 2 ELISA Kit; IBP-2 ELISA Kit; IBP2 ELISA Kit; IBP2_HUMAN ELISA Kit; IGF binding protein 2 ELISA Kit; IGF BP53 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP 2 ELISA Kit; IGFBP-2 ELISA Kit; IGFBP2 ELISA Kit; IGFBP53 ELISA Kit; Insulin like growth factor binding protein 2 36kDa ELISA Kit; Insulin like growth factor binding protein 2 ELISA Kit; Insulin like growth factor-binding protein 2 precursor ELISA Kit; Insulin-like growth factor-binding protein 2 ELISA Kit
缩写：IGFBP2
Uniprot No.：P18065
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-101 | ||||
| EDTA plasma (n=4) | 94 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.9352.9522.9442.767 252.0262.0762.0511.874 12.51.0881.0461.0670.890 6.250.5920.5830.5880.411 3.120.3610.3370.3490.172 1.560.2780.2590.2690.092 0.780.2320.2290.2310.054 00.1760.1770.177 | ||||||
货期：3-5 working days
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