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Human Macrophage Colony-Stimulating Factor Receptor,M-CSFR ELISA Kit

  • 中文名： 人巨噬细胞集落刺激因子受体(M-CSFR)ELISA Kit
货号: IPD-E10012h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人巨噬细胞集落刺激因子受体(M-CSFR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF1R含量。CSF1R是集落刺激因子1受体。它在巨噬细胞的生存、增殖和分化中发挥关键作用。针对它的研究聚焦于其信号通路，通过抑制CSF1R来调节巨噬细胞功能，有望用于肿瘤、自身免疫病等疾病治疗，为新疗法开发提供了方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。适用于科研领域中对巨噬细胞相关信号通路、免疫调控机制或疾病模型（如肿瘤、自身免疫性疾病）的体外蛋白表达分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C FMS ELISA Kit; CD 115 ELISA Kit; CD115 ELISA Kit; CD115 antigen ELISA Kit; CFMS ELISA Kit; Colony stimulating factor 1 receptor ELISA Kit; Colony stimulating factor I receptor ELISA Kit; CSF 1 R ELISA Kit; CSF 1R ELISA Kit; CSF-1 receptor ELISA Kit; CSF-1-R ELISA Kit; CSF1 R ELISA Kit; CSF1R ELISA Kit; CSF1R_HUMAN ELISA Kit; CSFR ELISA Kit; EC 2.7.10.1 ELISA Kit; FIM 2 ELISA Kit; FIM2 ELISA Kit; FMS ELISA Kit; FMS proto oncogene ELISA Kit; FMS protooncogene ELISA Kit; HDLS ELISA Kit; M-CSF Receptor ELISA Kit; M-CSF-R ELISA Kit; Macrophage colony stimulating factor 1 receptor ELISA Kit; Macrophage colony stimulating factor I receptor ELISA Kit; Macrophage colony-stimulating factor 1 receptor ELISA Kit; McDonough feline sarcoma viral (v fms) oncogene homolog ELISA Kit; MCSFR ELISA Kit; Oncogen FMS ELISA Kit; Proto-oncogene c-Fms ELISA Kit; V-FMS McDonough feline sarcoma viral oncogen homolog; formerly ELISA Kit
  • 缩写：
    CSF1R
  • Uniprot No.：
    P07333
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human M-CSFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:20

    Average %

    90


    Range %

    87-93


    1:40

    Average %

    102


    Range %

    98-106


    1:80

    Average %

    85


    Range %

    82-89


    1:160

    Average %

    96


    Range %

    93-99


  • 回收率：

    The recovery of human M-CSFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    86-100


    EDTA plasma (n=4)

    88

    84-93


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.908

    2.869

    2.889

    2.793


    5

    1.964

    1.932

    1.948

    1.852


    2.5

    1.115

    1.201

    1.158

    1.062


    1.25

    0.638

    0.642

    0.640

    0.544


    0.625

    0.336

    0.352

    0.344

    0.248


    0.312

    0.218

    0.220

    0.219

    0.123


    0.156

    0.142

    0.149

    0.146

    0.050


    0

    0.098

    0.093

    0.096





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