Mouse Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

中文名： 小鼠巨噬细胞集落刺激因子(M-CSF)ELISA Kit

货号: IPD-E04659m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠巨噬细胞集落刺激因子(M-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF1含量。CSF1，即巨噬细胞集落刺激因子（M-CSF），是一种分泌型细胞因子，能促进造血干细胞分化为巨噬细胞。CSF1通过其受体CSF1R影响巨噬细胞和单核细胞的功能，如吞噬、趋化活性及肿瘤细胞毒性。研究显示，CSF1在免疫、代谢和生育等生理过程中发挥重要作用，其信号通路在肿瘤和炎症等疾病中具有潜在治疗价值。试剂盒检测范围为7.81 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。适用于体外实验中对小鼠疾病模型（如炎症反应、自身免疫性疾病或肿瘤研究）的生物样本进行M-CSF表达动力学分析，为探索巨噬细胞相关调控机制及信号通路研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Csf1 ELISA kit; CsfmMacrophage colony-stimulating factor 1 ELISA kit; CSF-1 ELISA kit; MCSF) [Cleaved into: Processed macrophage colony-stimulating factor 1] ELISA kit
缩写：
CSF1
Uniprot No.：
P07141
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
7.8 pg/mL-500 pg/mL
灵敏度：
1.95 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)65.57562.15662.87663.70260.21663.763SD0.0380.0410.0440.0480.0520.055CV(%)4.2514.7625.0695.4796.1756.276

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse M-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

85-89

1:2

Average %

102

Range %

99-105

1:4

Average %

Range %

96-100

1:8

Average %

Range %

90-94

回收率：

The recovery of mouse M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-91
EDTA plasma (n=4)	95	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.844	2.966	2.905	2.758
250	2.065	2.102	2.084	1.937
125	1.433	1.382	1.408	1.261
62.5	0.794	0.861	0.828	0.681
31.2	0.538	0.544	0.541	0.394
15.6	0.309	0.332	0.321	0.174
7.8	0.234	0.231	0.233	0.086
0	0.143	0.150	0.147